Determination of Bisphenols, Bisphenol A Diglycidyl Ether (BADGE), BADGE Chlorohydrins and Hydrates from Canned Beer by High-Performance Liquid Chromatographytandem Mass Spectrometry.

Abstract

A set of 9 bisphenolic compounds has been analyzed from canned beer after liquid-liquid extraction (LLE). Namely bisphenol?A (BPA), bisphenol?F (BPF), bisphenol?S (BPS), bisphenol?A diglycidyl ether (BADGE), BADGE?2H2O, BADGE?H₂O, BADGE?HCl?H₂O, BADGE?2HCl, and BADGE?HCl have been examined. Stable isotope dilution assay (SIDA) using high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) was applied. Spiking and extraction experiments from Pilsner, wheat, and black beer resulted in recoveries from 75 to 118?%. For application experiment, 14 canned beer samples have been examined for bisphenolic content. Lead substances BPA and BADGE?2H₂O were tested positive in 14 out of 14 samples. Concentration ranges varied from 0.10 to 2.54??g/l and 0.64 to 14.3??g/l, respectively. Concentrations of BADGE?2H₂O were herein in accordance with the specific migration limit set by the European Union. For BPA and BADGE?2H₂O dietary intakes were calculated using the highest concentrations determined. The resulting daily intakes amounted to below 1?% of the current tolerable daily intakes. It can thus be concluded, that the examined set of canned beers can be considered to be safe for the consumer.