Supplementation to the MRS medium for the cultivation of fastidious beer spoilage bacteria

Authors

  • André Gillet
  • Marie-Hélène Dupuche
  • Nicolas Velings

Keywords:

Culture media, MRS, NBB, beer spoilage bacteria, detection procedures, Lactobacillus, Pediococcus

Abstract

The rapid detection and cultivation of the specific beer spoilage bacteria has always been a problem and still unresolved for some of the most fastidious Lactobacillus and Pediococcus species. This paper presents a new medium based on a adaptation of the MRS medium proposed by DeMan, Rogosa & Sharpe in 1960. The new medium is compared to others in term of size of the colonies on plates and kinetic of growth in liquid media. The kinetic is followed by optical density as well as cfu enumerations. In conclusion, we can say that with the new medium, we can easily cultivate and count some of the most fastidious bacterial beer spoilers.

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Published

2003-02-28

Issue

Vol. 56 No. 1/2 (2003): Monatsschrift für Brauwissenschaft

Section

Articles

License

Copyright (c) 2003 BrewingScience

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

Gillet, A., Dupuche, M.-H., & Velings, N. (2003). Supplementation to the MRS medium for the cultivation of fastidious beer spoilage bacteria. BrewingScience, 56(1/2), 10-14. https://brewingscience.de/index.php/brewingscience/article/view/584