Carbohydrate determination in wort and beer by HPLC-ELSD
Keywords:
Carbohydrates, wort, beer, oligosaccharides, HPLC-ELSDAbstract
Complete separation of fermentable and non fermentable carbohydrates of up to 15 glucose units in wort, mashed malt and beer was obtained in 40 minutes by direct injection on a NH₂ column and an HPLC-ELSD apparatus. The results were comparable with those found with the official HPLC-RI procedure and with chemical determination with Fehling's reagent. In Italian wort and mashed malt, about 88% of the total carbohydrates are fermentable, while in beer only 29% are fermentable.
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