Comparison of GFFF and LALLS methods for determination of large and small starch granules in spring barley grain (Hordeum vulgare L.)

Abstract

A newly developed method GFFF (Gravitational Field-Flow Fractionation) and the well known method LALLS (Low Angle Laser Light Scattering) were used to assess content of starch granules in the grain endosperm of seven varieties of spring barley (Hordeum vulgare L.). As a distribution criterion, the ratio of starch granules larger than 8 μm (type A) and smaller than 8 μm (type B) was chosen. Both methods assigned the varieties Akcent and Forum to the variety set with the highest ratio of large and small starch granules. Other studied varieties (Amulet, Kompakt, Olbram, Scarlett, and Tolar) formed a statistically indiscernible variety set with a lower ratio of large and small starch granules. Statistically highly significant correlation between the GFFF and LALLS methods was proved (r = 0.782).